Recombinant MEK1/MAP2K1 Monoclonal Antibody

SKU: AN300507P

Size::

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Recombinant Antibodies;Monoclonal Antibodies

Abbreviation:MAP2K1

Target Synonym:Mek;MEKK;Prkmk;MAP2K;MAPKK;MAPKK1;MEKK1;Mek1;Prkmk1;Map2k1

Research Areas:

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Mouse

Application:WB;ICC/IF

Isotype:IgG

Clonality:Monoclonal

Clone NO.:8C11

UNIProt ID:P31938

Accession:

Background:MEK1, also known as MAP2K1 and MKK1, is a member of the dual-specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. MEK1 is widely expressed, with extremely low levels in the brain. It lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon a wide variety of extra- and intracellular signals. As an essential component of the MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation, and development. Binding extracellular ligands such as growth factors, cytokines, and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1 and MEK2. MEK1 has been shown to export PPARG from the nucleus. The MAPK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. MKK1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Defects in MEK1 can cause Cardiofaciocutaneous Syndrome.

Concentration:1 mg/mL

Immunogen:Recombinant Mouse MEK1/MAP2K1 Protein

Buffer:0.2 μm filtered solution in PBS

Purification method:Protein A

Dilution:WB 1:500-1:2000;ICC/IF 1:20-1:100

Calculated MW:43 kDa

ObservedMW:43 kDa