Recombinant Klebsiella pneumoniae NEO Protein

SKU: PKSQ050062

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: NEO

Target Synonym: APH(3')-II;APH(3')II;Aminoglycoside 3'-phosphotransferase;Kanamycin kinase type II;Neomycin-kanamycin phosphotransferase type II;neo

Research Areas:

Conjugation:

Target Species: Klebsiella pneumoniae

Expression Host: E.coli

Application:

Fusion tag: None

UNIProt ID: P00552

Accession: P00552

Background: Aminoglycoside 3'-phosphotransferase (APH(3')), also known as aminoglycoside kinase, is an aminoglycoside-modifying enzyme and widely presented in resistant bacteria. These ATP-dependent enzymes phosphorylate the 3'-hydroxyl of a variety of aminoglycosides including kanamycins, neomycins, paromomycins, neamine, ribostamycin, geneticin, and paromamine. These phosphorylated aminoglycosides fail to bind to their respective ribosomal binding sites with high affinity; hence resistance is conferred to the drugs that are phosphorylated. APH(3') is primarily found in certain species of gram-positive bacteria.

Concentration:

Activity: Not validated for activity

Sequence: Met1-Phe264

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 6%Trehalose, 4%Mannitol, 0.05%Tween 80, PH8.0.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 29 kDa

ObservedMW: 26-30 kDa