Recombinant Human VWFCP/ADAMTS13 protein (His tag)

SKU: PDEH100888

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:VWFCP;ADAMTS13

Target Synonym:ADAM-TS 13;ADAMTS 13;ATS13;C9orf8;TTP;Von Willebrand factor cleaving protease;vWF CP;vWF-cleaving protease

Research Areas:Signal Transduction;Cancer;Cell Biology

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:N-His

UNIProt ID:Q76LX8

Accession:Q76LX8

Background:Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation.Zinc and calcium ions cooperatively modulate enzyme activity. The cleavage of the pro-domain is not required for protease activity. Dependence on calcium for proteolytic activity is mediated by the high affinity site.

Concentration:

Activity:Not validated for activity

Sequence:Thr 1151-Thr 1427

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:30.4 kDa

ObservedMW:31 kDa