Recombinant Human VEGF165 protein(His Tag)

SKU: PKSH034126

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: VEGF165

Target Synonym: Folliculostellate cell-derived growth factor;Glioma-derived endothelial cell mitogen;VPF

Research Areas:

Conjugation:

Target Species: Human

Expression Host: E.coli

Application: Cell culture

Fusion tag: C-His

UNIProt ID: P15692-4

Accession: NP_001165097

Background: Vascular endothelial growth factor (VEGF); also known as vascular permeability factor (VPF) and VEGF-A; is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects; including mediating increased vascular permeability; inducing angiogenesis; vasculogenesis and endothelial cell growth; promoting cell migration; inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability; thus it was originally referred to as vascular permeability factor.

Concentration:

Activity: Measure by its ability to induce HUVEC cells proliferation. The ED50 for this effect is < 5 ng/mL. The specific activity of recombinant human VEGF165 is approximately > 1. 4 x 10⁶IU/mg.

Sequence: Ala 27-Arg 191

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS,pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 20.1 kDa

ObservedMW: 21 kDa