Recombinant Human UBE2T Protein (His Tag)

SKU: PKSH033331

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: UBE2T

Target Synonym: Cell Proliferation-Inducing Gene 50 Protein;UBE2T;Ubiquitin Carrier Protein T;Ubiquitin-Conjugating Enzyme E2 T;Ubiquitin-Protein Ligase T

Research Areas: Cell biology;epigenetics and nuclear signal

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: N-His

UNIProt ID: Q9NPD8

Accession: Q9NPD8

Background: Ubiquitin-Conjugating Enzyme E2 T (UBE2T) is a ligase that belongs to the Ubiquitin-Conjugating Enzyme family. UBE2T accepts the ATP-dependent ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro; UBE2T is able to catalyze polyubiquitination using all 7 ubiquitin Lys residues; but may prefer 'Lys-11'-; 'Lys-27'-; 'Lys-48'- and 'Lys-63'-linked polyubiquitination. UBE2T is an important factor of the Faconi anemia pathway of DNA damage repair and; upon self-inactivation; may negatively regulate the Faconi pathway.

Concentration:

Activity: Not validated for activity

Sequence: Met 1-Val197

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 24.7 kDa

ObservedMW: 25-30 kDa