Recombinant Human UBE2D4 Protein (GST Tag)

SKU: PKSH033309

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: UBE2D4

Target Synonym: HBUCE1;UBCH5D;UBE2D4;Ubiquitin Carrier Protein D4;Ubiquitin-Conjugating Enzyme E2 D4;Ubiquitin-Protein Ligase D4

Research Areas: Cell biology;epigenetics and nuclear signal

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: N-GST

UNIProt ID: Q9Y2X8

Accession: Q9Y2X8

Background: Ubiquitin-Conjugating Enzyme E2 D4 (UBE2D4) is a ligase that belongs to the Ubiquitin-Conjugating Enzyme family. UBE2D4 has been proposed to participate in Ubl conjugation pathway. UBE2D4 takes part in post-translational protein modification; protein K6-linked ubiquitination; protein K11-linked ubiquitination; protein K27-linked ubiquitination; protein K29-linked ubiquitination; protein K48-linked ubiquitination; and protein K63-linked ubiquitination. UBE2D4 regulate of protein metabolic process. UBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro; UBE2D4 able to promote polyubiquitination using all 7 ubiquitin Lys residues; but may prefer 'Lys-11' and 'Lys-48'-linked poly-ubiquitination.

Concentration:

Activity: Not validated for activity

Sequence: Met 1-Met 147

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 43.5 kDa

ObservedMW: 40 kDa