Recombinant Human Transforming Growth Factor Beta-1/TGFB1 Biotinylated

SKU: PKSH034059

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: TGFB1

Target Synonym: LAP;Latency-Associated Peptide;TGF-Beta-1;TGFB;TGFB1;Transforming Growth Factor Beta-1

Research Areas: Signal Transduction;Cell biology;Cardiovascular;Cancer;metabolism;Stem cells

Conjugation: Biotinylated

Target Species: Human

Expression Host: HEK293 Cells

Application: Cell culture

Fusion tag: N-Avi

UNIProt ID: P01137

Accession: P01137

Background: Transforming Growth Factor β-1 (TGFβ-1) is a secreted protein which belongs to the TGF-β family. TGFβ-1 is abundantly expressed in bone, articular cartilage and chondrocytes and is increased in osteoarthritis (OA). TGFβ-1 performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. The precursor is cleaved into a latency-associated peptide (LAP) and a mature TGFβ-1 peptide. TGFβ-1 may also form heterodimers with other TGFβ family members. It has been found that TGFβ-1 is frequently upregulated in tumor cells. Mutations in this gene results in Camurati-Engelmann disease.

Concentration:

Activity: Measured by its ability to inhibit TGF-beta 1 activity on TF‑1 human erythroleukemic cells.The ED₅₀ for this effect is 137.37pg/ml.

Sequence: Ala279-Ser390

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 50mM Glycine-HCl, 150mM NaCl, pH2.5.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.

Calculated MW: 14.6 kDa

ObservedMW: 12-18 kDa