Recombinant Human TIM-3/HAVCR2 Protein (His Tag)

SKU: PKSH033509

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Immune Checkpoint Proteins

Abbreviation: TIM-3;HAVCR2

Target Synonym: HAVCR2;HAVcr-2;Hepatitis A Virus Cellular Receptor 2;T-Cell Immunoglobulin and Mucin Domain-Containing Protein 3;T-Cell Membrane Protein 3;TIM-3;TIM3;TIMD-3;TIMD3

Research Areas: immunology

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion tag: C-His

UNIProt ID: Q8TDQ0

Accession: AAL65157.1

Background: T-Cell Membrane Protein 3 (TIM3) is a single-pass type I membrane protein that belongs to the TIM family of immunoglobulin superfamily. TIM3 includes a signal sequence (aa 1-21); an extracellular region (aa 22-202) with one Ig-like V-type domain; a transmembrane segment (aa 203-223); and a cytoplasmic domain (aa 224 - 301). TIM3 regulates macrophage activation; inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. It may be also involved in T-cell homing and as a receptor for LGALS9.

Concentration:

Activity: Immobilized Anti-Human TIM-3 mAb at 2μg/ml (100 μl/well) can bind Human TIM-3-His. The ED50 of Recombinant Human TIM-3-His is 8.51ng/ml.

Sequence: Ser22-Arg200

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 20.9 kDa

ObservedMW: 37 kDa