Recombinant Human TGF alpha protein(His Tag)

SKU: PKSH034146

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: TGF alpha

Target Synonym: ETGF;Sarcoma growth factor;TGF-type I

Research Areas: Cardiovascular,Signal Transduction,Cancer

Conjugation:

Target Species: Human

Expression Host: E.coli

Application: Cell culture

Fusion tag: C-His

UNIProt ID: P01135

Accession: P01135

Background: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

Concentration:

Activity: Measure by its ability to induce 3T3 cells proliferation.The ED₅₀ for this effect is <0.2 ng/mL.The specific activity of recombinant human TGF alpha is > 5 x 10⁶ IU/mg.

Sequence: Val 40-Ala 89

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS,pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 6.5 kDa

ObservedMW: 7 kDa