Recombinant Human SRC Kinase/c-SRC Protein (His & GST Tag)

SKU: PKSH030385

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: ASV;SRC1;c-SRC;p60-Src

Research Areas: Signal Transduction,Epigenetics and Nuclear Signaling,Cancer

Conjugation:

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Application: Enzyme

Fusion tag: N-His-GST

UNIProt ID: P12931

Accession: P12931-1

Background: Proto-oncogene tyrosine-protein kinase SRC is a hydrophobic protein belonging to the SRC family kinase including nine members that is a family of non-receptor tyrosine kinases. SRC protein may exist in different forms: C-SRC and V-SRC. C-SRC is only activated under certain circumstances where it is required such as growth factor signaling, while V-SRC is a constitutively active as opposed to normal SRC (C-SRC). Thus, V-SRC is an instructive example of an oncogene protein kinase whereas C-SRC is a proto-oncogene protein kinase. Inhibition of SRC with NR2A tyrosine phosphorylation mediated by PSD-95 may contribute to the lithium-induced downregulation of NMDA receptor function and provide neuroprotection against excitotoxicity.

Concentration:

Activity: The specific activity was determined to be > 80 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.

Sequence: Met 1-Leu 536

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Supplied as sterile solution of 50mM Tris, 100mM NaCl, pH 8.0, 20% glycerol, 0.3mM DTT

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 87.7 kDa

ObservedMW: 81 kDa