Recombinant Human SCF protein

SKU: CD00965

Size:2 ug

Background:Stem Cell Factor (SCF) that binds to the c-Kit receptor is produced by fibroblasts and endothelial cells. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same RNA transcript and the presence of both soluble and transmembrane SCF is required for normal hematopoietic function. SCF plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. It also promotes mast cell adhesion, migration, proliferation, and survival. Human SCF shares 79 % - 87 % a.a. sequence identity with canine, feline, mouse, and rat SCF. Furthermore, human SCF is weakly active on mouse cells.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Stem Cell Factor

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using human TF-1 cells is less than 2 ng/ml, corresponding to a specific activity of > 5. × 15 IU/mg.

Synonyms:KITLG,DCUA, DFNA69, FPH2, FPHH, KL-1, Kitl, MGF, SCF, SF, SHEP7, SLF, Human

Endotoxin:Less than 1 EU/μg of rHuSCF as determined by LAL method.

Storage:-20ºC

Sequence:EGICRNRVTN NVKDVTKLVA NLPKDYMITL KYVPGMDVLP SHCWISEMVV QLSDSLTDLL DKFSNISEGL SNYSIIDKLV NIVDDLVECV KENSSKDLKK SFKSPEPRLF TPEEFFRIFN RSIDAFKDFV VASETSDCVV SSTLSPEKDS RVSVTKPFML PPVA

uniprot accession:P21583

Molecular weight:Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 164 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.