Recombinant Human ROR1 Protein (His Tag)

SKU: PKSH033647

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: ROR1

Target Synonym: Inactive tyrosine-protein kinase transmembrane receptor ROR1;NTRKR1;Neurotrophic tyrosine kinase;ROR1;receptor-related 1

Research Areas: Signal Transduction;Neuroscience;

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: Q01973

Accession: Q01973

Background: Receptor tyrosine kinase-like orphan receptor 1 (ROR1); also known as neurotrophic tyrosine kinase; it is a member of the ROR family within receptor tyrosine kinases (RTK) superfamily. Human ROR1 is a type I transmembrane protein with 937 amino acids (aa) in length. It contains a 29 aa signal sequence; a 377 aa extracellular domain (ECD); a 21 aa transmembrane segment; and a 510 aa cytoplasmic region. ROR1 expressed strongly in human heart; lung and kidney; but weakly in the CNS. At developmental stage; it expressed at high levels during early embryonic development. ROR1 has been shown to have very low kinase activity in vitro and is unlikely to function as a tyrosine kinase in vivo. It may act as a receptor for wnt ligand WNT5A which may result in the inhibition of WNT3A-mediated signaling.

Concentration:

Activity: Not validated for activity

Sequence: Gln30-Glu403

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 42.8 kDa

ObservedMW: 60-80 kDa