Recombinant Human RHEB Protein (His Tag)

SKU: PKSH031137

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: GTP-Binding Protein Rheb;RHEB;RHEB2;Ras Homolog Enriched in Brain

Research Areas: Signal Transduction;Cancer;metabolism;

Conjugation:

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Application:

Fusion tag: N-His

UNIProt ID: Q15382

Accession: Q15382

Background: RHEB is a recently discovered member of the Ras superfamily that may be involved in neural plasticity. This function is novel and not typically associated with the Ras proteins. RHEB gene is a member of the small GTPase superfamily and encodes a lipid-anchored; cell membrane protein with five repeats of the RAS-related GTP-binding region. RHEB is vital in regulation of growth and cell cycle progression due to its role in the insulin / TOR / S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form; and farnesylation of RHEB is required for this activity. Three pseudogenes have been mapped; two on chromosome 10 and one on chromosome 22.

Concentration:

Activity: Not validated for activity

Sequence: Met 1-Cys181

Purity: > 85 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 22.4 kDa

ObservedMW: 21 kDa