Recombinant Human RANKL/TNFSF11 Protein

SKU: PKSH031027

Size: 500μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: CD254;ODF;OPGL;OPTB2;Osteoclast differentiation factor;RANKL;Receptor activator of nuclear factor kappa-B ligand;TNFSF11;hRANKL2;sOdf;tumor necrosis factor ligand superfamily member 11

Research Areas: Immunology,Cardiovascular

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: Cell culture;ELISA

Fusion tag: None

UNIProt ID: O14788

Accession: AAC51762.1

Background: Tumor necrosis factor ligand superfamily member 10 (TNFSF10), also known as TNF-related apoptosis-inducing ligand (TRAIL), Apo-2 ligand, and CD253, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TNFSF10 functions as a ligand that induces the process of cell death called apoptosis. TNFSF10 shows homology to other members of the tumor necrosis factor superfamily. As one member of the cluster of differentiation system, TNFSF10 is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion TNFSF10 binds to several members of TNF receptor superfamily including TNFRSF10A / TRAILR1, TNFRSF10B / TRAILR2, TNFRSF10C / TRAILR3, TNFRSF10D / TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of TNFSF10 / TRAIL may be modulated by binding to the decoy receptors TNFRSF10C / TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8 / JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Concentration:

Activity: 1. Immobilized human TNFSF11 at 2 μg/ml (100 μl/well) can bind biotinylated human Osteoprotegerin hFc, The EC50 of human Osteoprotegerin hFc is 5-40 ng/ml.
2. The bioactivity of hRANKL was determined by measuring the ability of hRANKL to induce TRAP activity in Raw 264. 7 cells. The ED₅₀ for this effect is typically 1.5-7.5 ng/mL.

Sequence: Gly 64-Asp 245

Purity: > 85 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 20.5 kDa

ObservedMW: 27 kDa