Recombinant Human PTN protein

SKU: CD01025

Size:5 ug

Background:Pleiotrophin and Midkine are structurally related heparin-binding neurotrophic factors, whose expression is developmentally regulated. The expression pattern of these neurotrophic factors suggests function in neurogenesis, cell migration, secondary organogenetic induction, and mesoderm epithelial interaction. The expression of PTN increases during the process of brain embryogenesis and reaches maximum levels at time of birth. The physiological roles of PTN and Midkine are largely unknown, but these neurotrophins have been implicated in the pathogenesis of neuroblastomas. Recombinant human Pleiotrophin is a 15.3 kDa protein containing 136 amino acid residues and 5 intra-molecular disulfide bonds. PTN is a highly conserved protein. The amino acid sequences of human, bovine, rat, and mouse PTN share > 98 % identity.

Research_area:Others

Purity:> 96 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Pleiotrophin

Biological activity:Fully biologically active when compared to standard. The biological activity was measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons, when neurons were plated on 96 well culture plates that had been pre-coated with 1 μl/well of a solution of 5-1 μg/ml rHuPTN.

Synonyms:PTN, HARP, HB-GAM, HBBM, HBGF-8, HBGF8, HBNF, HBNF-1, NEGF1, OSF-1,Human

Endotoxin:Less than 0.1 EU/μg of rHuPTN as determined by LAL method.

Storage:-20ºC

Sequence:GKKEKPEKKV KKSDCGEWQW SVCVPTSGDC GLGTREGTRT GAECKQTMKT QRCKIPCNWK KQFGAECKYQ FQAWGECDLN TALKTRTGSL KRALHNAECQ KTVTISKPCG KLTKPKPQAE SKKKKKEGKK QEKMLD

uniprot accession:P21246

Molecular weight:Approximately 15.3 kDa, a single non-glycosylated polypeptide chain containing 136 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered solution in PBS, pH 7.4.