Recombinant Human POMGNT1 Protein (His Tag)

SKU: PKSH032917

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: POMGNT1

Target Synonym: 2-N-Acetylglucosaminyltransferase 1;2-N-Acetylglucosaminyltransferase I.2;MGAT1.2;POMGNT1;POMGnT1;Protein O-Linked-Mannose Beta-1;UDP-GlcNAc:Alpha-D-Mannoside Beta-1

Research Areas: Tags & Cell Markers

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: Q8WZA1

Accession: Q8WZA1

Background: Protein O-Linked-Mannose β-1 2-N-Acetylglucosaminyltransferase 1 (POMGNT1) belongs to the Glycosyltransferase 13 family. Amino acid residues between 299-311 are important for both protein expression and enzymatic activity. The minimal catalytic domain is located between positions 299-651. It is suggested that the stem domain of the soluble form is unnecessary for activity, but that some amino acids play a crucial role in the membrane-bound form. Defects in POMGNT1 are the cause of muscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies type A3 (MDDGA3).

Concentration:

Activity: Not validated for activity

Sequence: Leu59-Thr660

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.5.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 69.3 kDa

ObservedMW: 74 kDa