Recombinant Human PKLR Protein (His Tag)

SKU: PKSH032984

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: PKLR

Target Synonym: PK1;PKL;PKLR;Pyruvate Kinase 1;Pyruvate Kinase Isozymes R/L;R-Type/L-Type Pyruvate Kinase;Red Cell/Liver Pyruvate Kinase

Research Areas: Signal Transduction;Cardiovascular;Cancer;epigenetics and nuclear signal;metabolism;

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: P30613

Accession: P30613

Background: Pyruvate Kinase Isozymes R/L (PKLR) belongs to the pyruvate kinase family, There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver; R is found in red cells; M1 is the main form in muscle, heart and brain; M2 is found in early fetal tissues. PKLR exists as a homotetramer and catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. Defects in PKLR are also the cause of pyruvate kinase deficiency of red cells, which is a frequent cause of hereditary non-spherocytic hemolytic anemia.

Concentration:

Activity: Not validated for activity

Sequence: Met 1-Ser574

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, 5% Trehalose, 5% Mannitol, 0.02% Tween 80, 50% Glycerol, 1mM EDTA, 1mM DTT, pH8.0.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 62.9 kDa

ObservedMW: 58 kDa