Recombinant Human PCSK9 Protein (D374Y, His Tag)

SKU: PKSH032947

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: PCSK9

Target Synonym: NARC-1;NARC1;Neural Apoptosis-Regulated Convertase 1;PC9;PCSK9;Proprotein Convertase 9;Proprotein Convertase Subtilisin/Kexin Type 9;Subtilisin/Kexin-Like Protease PC9

Research Areas: Signal Transduction;Cell biology;Cardiovascular;Cancer;metabolism;Stem cells

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion tag: C-His

UNIProt ID: Q8NBP7

Accession: Q8NBP7

Background: Recombinant Human Proprotein Convertase Subtilisin/Kexin Type 9/PCSK9 (D374Y) is a gain of function mutant of human PCSK9 protein. Human PCSK9 is a secretory subtilase belonging to the proteinase K subfamily. PCSK9 is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the ER, the pro domain and mature chain are secreted together through noncovalent interactions. PCSK9 binds with low-density lipoprotein receptor (LDLR) and it plays a major regulatory role in cholesterol homeostasis. Inhibition of PCSK9 function by preventing PCSK9/LDLR interaction is currently being explored as a means of lowering cholesterol levels. PCSK9 also binds to apolipoprotein receptor 2 (ApoER2), and play a role in the neural development.

Concentration:

Activity: Immobilized Recombinant Human LDL R (C-Fc) at 5μg/ml (100 μl/well) can bind Recombinant Human PCSK9 . The ED₅₀ of Recombinant Human PCSK9 is 39.17 ng/ml.

Sequence: Gln31-Gln692(Asp374Tyr,Val474Ile,Gly504Arg,Gly670Glu)

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 20% Glycerol, pH 7.4.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 15-18&60-80 kDa

ObservedMW: 13.8&58.2 kDa