Recombinant Human NFYA Protein (GST Tag)

SKU: PKSH032825

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: NFYA

Target Synonym: CAAT Box DNA-Binding Protein Subunit A;NF-YA;NFYA;Nuclear Transcription Factor Y Subunit A;Nuclear Transcription Factor Y Subunit Alpha

Research Areas: Epigenetics and Nuclear Signaling

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: N-GST

UNIProt ID: P23511

Accession: P23511-2

Background: Nuclear Transcription Factor Y Subunit α (NFYA) is a member of the NFYA/HAP2 subunit family. NFYA founctions as a heterotrimeric transcription factor ; which is composed of three components; NF-YA; NF-YB and NF-YC; binds to CCAAT motifs in the promoter regions in a variety of genes. NFYA forms a highly conserved transcription factor which stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters; for example in type 1 collagen; albumin and beta-actin genes.

Concentration:

Activity: Not validated for activity

Sequence: Met 1-Ser318

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 60.6 kDa

ObservedMW: 55 kDa