Recombinant Human MLP3B/MAP1LC3B protein (His tag)

SKU: PDEH100897

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:MLP3B;MAP1LC3B

Target Synonym:ATG8F;Autophagy related protein LC3 A;Autophagy related ubiquitin like modifier LC3 A;Autophagy related ubiquitin like modifier LC3 B;LC3;MAP1 light chain 3 like protein 1;MAP1 light chain 3 like protein 2;MAP1A/1B light chain 3 A;MAP1A/1BLC3;MAP1AL;MLP3B

Research Areas:Signal Transduction;Cardiovascular;Cancer;Neuroscience;Metabolism

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:N-His

UNIProt ID:Q9GZQ8

Accession:Q9GZQ8

Background:Ubiquitin-like modifier involved in formation of autophagosomal vacuoles. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria. While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.

Concentration:

Activity:Not validated for activity

Sequence:Met 1-Val 125

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:13.6 kDa

ObservedMW:18 kDa