Recombinant Human Merlin/NF2 protein (His Tag)

SKU: PDEH100698

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:Merlin;NF2

Target Synonym:BANF;Bilateral acoustic neuroma;CAN;MERL_HUMAN;Merlin;Moesin ezrin radixin like protein;Moesin ezrin radizin like;Moesin-ezrin-radixin-like;NF 2;Neurofibromatosis 2;Neurofibromatosis type 2;Neurofibromatosis2;SCH;Schwannomerlin

Research Areas:Neuroscience,Signal Transduction,Epigenetics and Nuclear Signaling,Cancer

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:N-His

UNIProt ID:P35240

Accession:P35240-1

Background:Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.

Concentration:

Activity:Not validated for activity

Sequence:Met1-Leu595

Purity: > 90% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4., 5% trehalose, 5% mannitol, 0.01% Tween 80.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:69.7 kDa

ObservedMW:68-71 kDa