Recombinant Human LYPLA1 Protein (His Tag)

SKU: PKSH032043

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: LYPLA1

Target Synonym: APT-1;APT1;Acyl-Protein Thioesterase 1;LPL-I;LPL1;LYPLA1;LysoPLA I;Lysophospholipase 1;Lysophospholipase I;hAPT1

Research Areas: Signal Transduction;Cancer;metabolism;

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: N-His

UNIProt ID: O75608

Accession: O75608

Background: Acyl-Protein Thioesterase 1 (APT-1) is lysophospholipase that belongs to the AB hydrolase 2 family. Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. APT-1 performs on biological membranes to regulate the multifunctional lysophospholipids. It hydrolyzes lysophosphatidylcholine in both monomeric and micellar forms. It hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS; in addition, it also has depalmitoylating activity and low lysophospholipase activity.

Concentration:

Activity: Not validated for activity

Sequence: Met 1-Asp230

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 10% Glycerol, pH 8.0.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 26.8 kDa

ObservedMW: 25 kDa