Recombinant Human LR3 IGF-1 protein

SKU: CD01368

Size:1 mg

Background:IGF-1 belonged to the insulin gene family, is a mitogenic polypeptide growth factor that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. It is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. The LR3 IGF-1 is a long-term analog of human IGF-1, specifically designed and manufactured for mammalian cell culture to support large-scale manufacturing of recombinant biopharmaceuticals.

Research_area:Immunology

Purity:>98 % by SDS-PAGE analyses.>90 % by RP-HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human LR3 Insulin-like Growth Factor-1

Biological activity:Assay 1: Fully biologically active when compared to standard. Measured in a serum-free cell proliferation assay using human MCF-7 cells. The ED5 for this effect is .3-1.5 ng/ml, corresponding to a specific activity of > 6.7 × 15 IU/mg.Assay 2: Fully biologically active when compared to standard. The ED5 as determined by the stimulation of protein synthesis using rat L6 myoblasts is less than 1 ng/ml, corresponding to a specific activity of > 1. × 15 IU/mg.

Synonyms:LR3 IGF-1, IGF, IGF-I, IGFI, MGF,Human

Endotoxin:Less than 0.01 EU/μg of rHuLR3 IGF-1 as determined by LAL method.

Storage:-20ºC

Sequence:MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA

uniprot accession:P519

Molecular weight:Approximately 9.1 kDa, a single non-glycosylated polypeptide chain containing 83 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 mM PB, pH 7.2.