Recombinant Human LC3A/B Protein(Trx Tag)

SKU: PDEH100633

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:LC3A/B

Target Synonym:MAP1A/1BLC;MAP1 light chain 3-like protein;MAP1ALC;MAP1LC3B;ATG8F;LC3B;MAP1A/1BLC3;MAP1LC3B-a;microtubule associated protein 1 light chain 3 beta;Autophagy-related protein LC3 B;Autophagy-related ubiquitin-like modifier LC3 B;LC3A/LC3B;MAP1 light chain 3-like protein 2;MAP1A/MAP1B LC3 B;MAP1A/MAP1B light chain 3 B;Microtubule-associated protein 1 light chain 3 beta;MAP1ALC3;Autophagy related protein LC3 A;Autophagy related ubiquitin like modifier LC3 A;Autophagy related ubiquitin like modifier LC3 B;LC3;LC3II;MAP;MAP1 light chain 3 like protein 1;MAP1 light chain 3 like protein 2;MAP1A/1B light chain 3 A;MAP1LC3B a;Microtubule-associated proteins 1A/1B light chain 3B;MLP3B

Research Areas:Signal Transduction;Neuroscience;Cardiovascular;Cancer;Metabolism

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:N-Trx

UNIProt ID:Q9GZQ8

Accession:Q9GZQ8

Background:LC3A, also known as MAP1LC3A, is one of the light chain subunits that function together with both MAP1A and/or MAP1B. MAP1A and MAP1B are microtubule-associated proteins that mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. As a light chain subunit, MAP1LC3A has an important part in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP1LC3A is expressed as two alternatively spliced isoforms that are expressed in testis, brain, heart, liver, and skeletal muscle but are absent in thymus and peripheral blood leukocytes.

Concentration:

Activity:Not validated for activity

Sequence:His27-Val125

Purity: > 90% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:30.7 kDa

ObservedMW:31 kDa