Recombinant Human ITPase/ITPA Protein (His Tag)

SKU: PKSH032588

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: ITPase;ITPA

Target Synonym: ITPase;Inosine Triphosphatase;Inosine Triphosphate Pyrophosphatase;NTPase;Non-Canonical Purine NTP Pyrophosphatase;Non-Standard Purine NTP Pyrophosphatase;Nucleoside-Triphosphate Diphosphatase;Nucleoside-Triphosphate Pyrophosphatase;Putative Oncogene Prot

Research Areas: Signal Transduction;Cancer;metabolism;

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: C-His

UNIProt ID: Q9BY32

Accession: Q9BY32

Background: Inosine Triphosphate Pyrophosphatase (ITPase) is a cytoplasmic enzyme that belongs to the HAM1 NTPase family. ITPase hydrolyzes the non-canonical purine nucleotides inosine triphosphate (ITP) and deoxyinosine triphosphate (dITP) to the monophosphate nucleotide (IMP) and diphosphate. The ITPase enzyme acts as a homodimer and does not distinguish between the deoxy- and ribose forms. ITPase probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions. Defects in ITPase is thought to be inherited and is characterized by an over-accumulation of ITP in erythocytes, leukocytes and fibroblasts.

Concentration:

Activity: Not validated for activity

Sequence: Ala2-Ala194

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 5% Trehalose, 300mM NaCl, 30% Glycerol, 0.05% Tween 80, pH8.0.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 22.5 kDa

ObservedMW: 21 kDa