Size:100ug. Other sizes are also available. Please contact us.
Research Areas:Immunology
Uniprot NO.:P22301
Uniprot Entry Name:IL10_HUMAN
Gene Names:IL10
Species:Homo sapiens (Human)
Source:E.Coli
Expression Region:19-178aa
Sequence:SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN
Protein Description:Full Length of Mature Protein
Tag Info:Tag-Free
Mol. Weight:18.6 kDa
Biological_Activity:Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1 ng/ml, corresponding to a specific activity of ? 1.0 × 106 IU/mg.
Purity:>95% as determined by SDS-PAGE and HPLC.
Endotoxin:Less than 1.0 EU/µg as determined by LAL method.
Form:Lyophilized powder
Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Alternative Name/ Alias:IL-10, CSIF
Relevance:Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
PubMed ID:1847510; 9405662; 15340161; 8364028; 14657427; 7547951; 8590020; 8897595; 11485736; 15837194; 12825869
Function:Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Involvement in disease:
Subcellular Location:Secreted
Protein Families:IL-10 family
Tissue Specificity:Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
Paythway:Jak-STATsignalingpathway
HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:5962
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=193717
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:3586
STRING Database Link:https://string-db.org/network/9606.ENSP00000412237
OMIM Database Link:https://www.omim.org/entry/124092124092124092