Size:100ug. Other sizes are also available. Please contact us.
Research Areas:Stem Cells
Uniprot NO.:Q14623
Uniprot Entry Name:IHH_HUMAN
Gene Names:IHH
Species:Homo sapiens (Human)
Source:E.Coli
Expression Region:29-202aa
Sequence:II+GPGRVVGS RRRPPRKLVP LAYKQFSPNV PEKTLGASGR YEGKIARSSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDRLNSLAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRNKYGLL ARLAVEAGFD WVYYESKAHV HCSVKSEHSA AAKTGG
Protein Description:Partial
Tag Info:Tag-Free
Mol. Weight:19.8 kDa
Biological_Activity:Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 3.0-10 ?g/ml.
Purity:>96% as determined by SDS-PAGE and HPLC.
Endotoxin:Less than 1.0 EU/µg as determined by LAL method.
Form:Lyophilized powder
Buffer:Lyophilized from a 0.2 ?m filtered 1 × PBS, pH 7.4
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Alternative Name/ Alias:IHH,
Relevance:Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP) (By similarity). {ECO:0000250}.
PubMed ID:15489334; 7590746; 7720571; 9593755; 16335952; 20519495; 21537345; 11455389; 12384778; 12632327
Function:Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification
Involvement in disease:Brachydactyly A1 (BDA1); Acrocapitofemoral dysplasia (ACFD)
Subcellular Location:Indian hedgehog protein N-product: Cell membrane, Lipid-anchor, Extracellular side, Note=The N-terminal peptide remains associated with the cell surface, SUBCELLULAR LOCATION: Indian hedgehog protein C-product: Secreted, extracellular space
Protein Families:Hedgehog family
Tissue Specificity:Expressed in embryonic lung, and in adult kidney and liver.
Paythway:Hedgehogsignalingpathway
HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:5956
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=654504
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:3549
STRING Database Link:https://string-db.org/network/9606.ENSP00000295731
OMIM Database Link:https://www.omim.org/entry/112500112500112500