Recombinant Human IL1F6/IL36A Protein (His Tag)

SKU: PKSH031509

Size: 20μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: IL1F6/IL36A

Target Synonym: FIL;IL1F;Interleukin-1 Family Member;IL-1F;IL36A;FIL1;FIL1(EPSILON);FIL1E;IL-1F6;IL1(EPSILON);IL1F6;FIL1 Epsilon;IL-1 Epsilon;Interleukin-1 Epsilon;Interleukin-1 Family Member 6;Interleukin-36 Alpha;IL1E;IL 1 epsilon;IL 1F6;IL 1H1;IL1RP2;Interleukin 1 epsilon;Interleukin 1 family member 6;Interleukin 1 family member 6 (epsilon);Interleukin 36 alpha;MGC129552;MGC129553;MGC151479;MGC151481;OTTMUSP00000012798;RP23-176J12.4

Research Areas: immunology

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: N-His

UNIProt ID: Q9UHA7

Accession: Q9UHA7

Background: Interleukin-1 family member 6 (IL-1F6); also known as interleukin 36; alpha (IL36A); is a pro-inflammatory cytokine which plays an important role in innate and adaptive immunity. IL-1F6 activates MAPK and NF-kB pathways and is produced by many different cells. This cytokine is a family member of interleukin-1 (IL-1) and plays an important role in the pathophysiology of several diseases. It has been reported that IL-1F6 and IL-1F8; in addition to IL-1F9; activate the pathway leading to NF-kappaB in an IL-1Rrp2-dependent manner in Jurkat cells as well as in multiple other human and mouse cell lines. Activation of the pathway leading to NF-kappaB by IL-1F6 and IL-1F8 follows a similar time course to activation by IL-1beta; suggesting that signaling by the novel family members occurs through a direct mechanism. In a mammary epithelial cell line; NCI/ADR-RES; which naturally expresses IL-1Rrp2; all three cytokines signal without further receptor transfection. IL-1Rrp2 antibodies block activation of the pathway leading to NF-kappaB by IL-1F6; IL-1F8; and IL-1F9 in both Jurkat and NCI/ADR-RES cells. Thus IL-1F6; IL-1F8; and IL-1F9 signal through IL-1Rrp2 and IL-1RAcP.

Concentration:

Activity: Not validated for activity

Sequence: Lys 6-Phe158

Purity: > 99 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: Please contact us for more information.

Calculated MW: 19.2 kDa

ObservedMW: 20 kDa