Recombinant Human IL-6/Interleukin-6 Protein

SKU: PKSH033611

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: IL-6;Interleukin-6

Target Synonym: IFNB;Interleukin HP;Interferon Beta;B-Cell Stimulatory Factor;IFN-Beta;B-cell hybridoma growth factor;B-Cell Stimulatory Factor 2;BSF2;BSF-2;CDF;CTL Differentiation Factor;HGF;HSF;Hybridoma Growth Factor;IFN-Beta-2;IL-6;Interferon Beta-2;Interleukin 6;Interleukin HP-1;Interleukin-6;IL6;IFNB2;B cell differentiation factor;B cell stimulatory factor 2;BSF 2;Hepatocyte stimulatory factor;Hybridoma growth factor Interferon beta-2;IL 6;Interferon beta 2;Interleukin 6 (interferon beta 2);Interleukin BSF 2;Interleukin-6;IL-6;B-cell hybridoma growth factor;Interleukin HP-1

Research Areas: Immunology,Neuroscience,Cancer,Metabolism

Conjugation:

Target Species: Human

Expression Host: E.coli

Application: Cell culture

Fusion tag: C-His

UNIProt ID: P05231

Accession: P05231

Background: Cytokines of the IL6/GCSF/MGF family are glycoproteins of about 170 to 180 amino acid residues that contain four conserved cysteine residues involved in two disulfide bonds. They have a compact, globular fold (similar to other interleukins), stabilized by the 2 disulfide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist; the helices are anti-parallel, with 2 overhand connections, which fall into a 2-stranded anti-parallel beta-sheet. The fourth alpha helix is important to the biological activity of the molecule. Interleukin-6 (IL-6) is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro.

Concentration:

Activity: 1. Measure by its ability to induce proliferation in TF-1 cells. The ED₅₀ for this effect is <0.5 ng/mL. The specific activity of recombinant human IL-6 is approximately >5 x 10⁸ IU/mg.
2. Measure by its ability to induce proliferation in MCF-7 cells. The ED₅₀ for this effect is <4.4 ng/mL.

Sequence: Val30-Met212

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS,pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 21.8 kDa

ObservedMW: 22 kDa