Recombinant Human IL-5 protein

SKU: CD01492

Size:2 ug

Background:The IL-5 gene is located on chromosome 5 in humans, in close proximity to the genes encoding IL-3, IL-4, and granulocyte-macrophage colony-stimulating factor (GM-CSF), which are often co-expressed in TH2 cells. Through binding to the IL-5 receptor, IL-5 stimulates B cell growth and increases immunroglobulin secretion. It is also a key mediator in eosinophil activation. Interleukin-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma. IL-5 is a 115-amino acid (in human, 133 in the mouse) -long TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely IL-3 and GM-CSF), this glycoprotein in its active form is a homodimer.

Research_area:Immunology

Purity:> 98 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Interleukin-5

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using human TF-1 cells is less than .1 ng/ml, corresponding to a specific activity of > 1. × 17 IU/mg.

Synonyms:IL-5, EDF, IL5, TRF,Human

Endotoxin:Less than 1 EU/μg of rHuIL-5 as determined by LAL method.

Storage:-20ºC

Sequence:MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES

uniprot accession:P5113

Molecular weight:Approximately 26.5 kDa, a disulfide-linked homodimeric protein containing two 116 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2mM PB, pH 7.5, 3 % trehalose, .1 % Tween-8.