Recombinant Human IL-34 protein(His Tag)

SKU: PKSH034117

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: IL-34

Target Synonym: Chromosome 16 Open Reading Frame;C16orf;Chromosome 16 Open Reading Frame 77;C16orf77;IL34

Research Areas: Immunology

Conjugation:

Target Species: Human

Expression Host: E.coli

Application: Cell culture

Fusion tag: C-His

UNIProt ID: Q6ZMJ4

Accession: Q6ZMJ4

Background: Interleukin-34 (IL-34) is a protein that promotes the proliferation,survival and differentiation of monocytes and macrophages. It also promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. IL-34 plays an important role in the regulation of osteoclast proliferation and differentiation,and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1.

Concentration:

Activity: Measure by its ability to induce human peripheral blood monocytes (PBMCs) proliferation. The ED₅₀ for this effect is <30 pg/mL.

Sequence: Asn 21-Pro 242

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS,pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 26.2 kDa

ObservedMW: 30 kDa