Recombinant Human IHH C28II protein

SKU: CD01574

Size:5 ug

Background:IHH, encoded by the IHH gene in humans, belongs to the mammalian hedgehog family. It is expressed in adult kidney and liver. The function of IHH is involved in chondrocyte differentiation, proliferation and maturation especially during endochondral ossification. It regulates its effects by feedback control of parathyroid hormone-related peptide (PTHrP). IHH is also involved in yolk sac vasculogenesis, playing an important role in differentiation of epiblast cells into endothelial and red blood cells. Human IHH shares 100 % amino acid sequence identity with Mouse.

Research_area:Others

Purity:> 96 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Indian Hedgehog Cys28IleIle

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by its ability to induce alkaline phosphatase production by C3H1T1/2(CCL-226) cells is 3.-1 μg/ml.

Synonyms:IHH C28II,BDA1, HHG2, Human

Endotoxin:Less than 1 EU/μg of rHuIHH C28II as determined by LAL method.

Storage:-20ºC

Sequence:IIGPGRVVGS RRRPPRKLVP LAYKQFSPNV PEKTLGASGR YEGKIARSSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDRLNSLAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRNKYGLL ARLAVEAGFD WVYYESKAHV HCSVKSEHSA AAKTGG

uniprot accession:Q14623

Molecular weight:Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 1 × PBS, pH 7.4.