Recombinant Human IGF-1/IGF1 Protein (aa 52-118)

SKU: PKSH032595

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: IGF-1;IGF1

Target Synonym: IBP1;IGF-I;IGF1;Insulin-Like Growth Factor I;MGF;Mechano Growth Factor;Somatomedin-C

Research Areas: Signal Transduction;Cancer;Developmental Biology;

Conjugation:

Target Species: Human

Expression Host: E.coli

Application: Cell culture

Fusion tag: None

UNIProt ID: P05019

Accession: P05019

Background: Insulin-like growth factor I (IGF1) belongs to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGFs are generated by proteolytic processing of inactive precursor protein containing N-terminal and C-terminal propeptide regions. Mature human IGF-I consisting of 70 amino acids with 94% identity with mouse IGF1 and exhibits cross-species activity. IGF1 binds IGF-1R; IGF-2R; and the insulin receptor and plays a key role in cell cycle progression; cell proliferation and tumor progression. IGF1 expression is regulated by growth hormone.

Concentration:

Activity: Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED₅₀ for this effect is 20-100 ng/ml.

Sequence: Thr52-Ala118

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM NaAc-HAc, pH 4.5
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.5 EU per μg of the protein as determined by the LAL method.

Calculated MW: 7.3 kDa

ObservedMW: 9 kDa