Recombinant Human IDO1/IDO Protein

SKU: PKSH030355

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Immune Checkpoint Proteins

Abbreviation:

Target Synonym: 3-dioxygenase;3-dioxygenase 1;IDO;IDO-1;IDO1;INDO;Indole 2;Indoleamine 2

Research Areas: Signal Transduction;Cardiovascular;Cancer;metabolism;

Conjugation:

Target Species: Human

Expression Host: E.coli

Application: Enzyme

Fusion tag: None

UNIProt ID: P14902

Accession: NP_002155.1

Background: Indoleamine 2,3-dioxygenase-1, also known as Indoleamine-pyrrole 2,3-dioxygenase, IDO1 and IDO, is a member of the indoleamine 2,3-dioxygenase family. IDO1 / IDO and tryptophan 2,3-dioxygenase (TDO) are tryptophan-degrading enzymes that catalyze the first step in tryptophan catabolism via the kynurenine pathway. TDO is widely distributed in both eukaryotes and bacteria. In contrast, IDO has been found only in mammals and yeast. In 2007, a third enzyme, indoleamine 2,3-dioxygenase-2 (IDO2), was discovered. IDO2 is found not only in mammals but also in lower vertebrates. IDO1 / IDO is an immunosuppressive molecule inducible in various cells. IDO1 / IDO catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. It mediates oxidative cleavage of tryptophan, an amino acid essential for cell proliferation and survival. IDO1 / IDO inhibition is proposed to have therapeutic potential in immunodeficiency-associated abnormalities, including cancer. The IDO pathway is activated in multiple tumor types. Selective inhibition of IDO1 may represent an attractive cancer therapeutic strategy via up-regulation of cellular immunity. IDO1 / IDO is an enzyme that suppresses adaptive T-cell immunity by catabolizing tryptophan from the cellular microenvironment. Inhibition of IDO pathway might enhance the efficacy of immunotherapeutic strategies for cancer

Concentration:

Activity: Measured by its ability to oxidize L-tryptophan to N-formylk-ynurenine. The specific activity is > 500 pmoles/min/μg.

Sequence: Ala2-Gly403

Purity: > 85 % as determined by reducing SDS-PAGE.

Formulation: Supplied as sterile solution of 50 mM NaAC, 100 mM Nacl, 20 % glycerol, pH 5.5.

Reconstitution: Not Applicable

Endotoxin: Please contact us for more information.

Calculated MW: 45.2 kDa

ObservedMW: 46 kDa