Recombinant Human HMGB1 protein (C-6His)

SKU: CD01646

Size:10 ug

Background:Human High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (a.a.) single chain polypeptide containing three domains: two N-terminal globular, 70 a.a. positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 a.a. C-terminal region that contains only Asp and Glu.4, 5 Residues 27 - 43 and 178 - 184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins.

Research_area:Others

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human High-Mobility Group Box 1, His

Biological activity:Data not available.

Synonyms:HMGB1, HMG-1, HMG1, HMG3, SBP-1, Human

Endotoxin:Less than 1 EU/μg of rHuHMGB1, His as determined by LAL method.

Storage:-20ºC

Sequence:MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDELEHHH HHH

uniprot accession:P9429

Molecular weight:Approximately 26. kDa, a single non-glycosylated polypeptide chain containing 223 amino acids with 6 × His at C-terminus.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.