Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase(GAPDH),partial

Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase(GAPDH),partial

CSB-RP000254h
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Size: 200ug. Other sizes are also available. Please Inquire.

In Stock: No

Lead time: 10-20 working days

Research Topic: Apoptosis

Uniprot ID: P04406

Gene Names: GAPDH

Organism: Homo sapiens (Human)

AA Sequence: KVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE

Expression Region: 3-335aa

Sequence Info: Partial

Source: E.coli

Tag Info: N-terminal GST-tagged

MW: 62.9 kDa

Alternative Name(s): Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-)

Relevance: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assbly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and mbrane associations through its ability to stimulate the binding of CHP1 to microtubules . Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assbles into the GAIT complex which binds to st loop-containing GAIT elents in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.3 Publications

Reference: The glyceraldehyde 3 phosphate dehydrogenase gene family structure of a human cDNA and of an X chromosome linked pseudogene; amazing complexity of the gene family in mouse.Hanauer A., Mandel J.-L.EMBO J. 3:2627-2633(1984)

Purity: Greater than 90% as determined by SDS-PAGE.

Storage Buffer: Tris-based buffer,50% glycerol

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

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