Recombinant Human Gastric Lipase/LIPF Protein (Human Cells, His Tag)

SKU: PKSH032480

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: Gastric Lipase;LIPF

Target Synonym: GL;Gastric Lipase;Gastric Triacylglycerol Lipase;HGL;HLAL;LIPF

Research Areas: Signal Transduction;Cardiovascular;Cancer;metabolism;

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: P07098

Accession: AAI12273.1

Background: Gastric Triacylglycerol Lipase (LIPF) belongs to the AB hydrolase superfamily. LIPF is an important lipase during the digestion of dietary lipids in cystic fibrosis. LIPF is involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. LIPF is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. LIPF acts distinct roles in neutral lipid metabolism.

Concentration:

Activity: Not validated for activity

Sequence: Leu20-Lys398

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 25mM Tris-HCl, 100mM glycine, 10% Glycerol, pH 7.3.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 44.2 kDa

ObservedMW: 50 kDa