Recombinant Human F9 Protein(His Tag)

SKU: PDMH100254

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:F9

Target Synonym:Christmas Disease;Christmas factor;Coagulant factor IX;Coagulation factor 9;Coagulation factor IX;Coagulation factor IXa heavy chain;F9;FA9;Factor 9;Factor IX Deficiency;FactorIX;FIX;Haemophilia B;HEMB;MGC129641;MGC129642;P19;Plasma Thromboplastic Component;Plasma thromboplastin component;PTC

Research Areas:Cardiovascular;Immunology

Conjugation:

Target Species:Human

Expression Host:stable cell line

Application:

Fusion tag:C-His

UNIProt ID:P00740

Accession:P00740

Background:This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with an Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing.

Concentration:

Activity:Not validated for activity

Sequence:Met1-Thr461

Purity: > 90% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 1.0 EU/mg of the protein as determined by the LAL method.

Calculated MW:50.6 kDa

ObservedMW:60-80 kDa