Recombinant Human EPO Receptor/EPOR Protein (Fc Tag)

SKU: PKSH031462

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: EPO-R

Research Areas: Signal Transduction;Cardiovascular;immunology;Stem cells

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: Cell culture;ELISA

Fusion tag: C-hFc

UNIProt ID: P19235

Accession: NP_000112.1

Background: Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis, and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR/EPO Receptor) on bone marrow erythroblasts, which transmits signals important for both proliferation and differentiation along the erythroid lineage. EPOR protein is a type â… single-transmembrane cytokine receptor, and belongs to the homodimerizing subclass which functions as ligand-induced or ligand-stabilized homodimers. EPOR signaling prevents neuronal death and ischemic injury. Recent studies have shown that EPO and EPOR protein may be involved in carcinogenesis, angiogenesis, and invasion.

Concentration:

Activity: 1. Immobilized CD131 at 10 μg/ml (100 μl/well) can bind biotinylated recombinant human EPOR with a linear range of 0.16-4 μg/ml. 2. Measured by its ability to inhibit Epo­dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 1-4 ng/ml in the presence of 0.1 U/mL Recombinant Human EPO.

Sequence: Met-Pro 250

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, 8% sucrose, 0.5% Tween-20, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 51.0 kDa

ObservedMW: 55-60 kDa