Recombinant Human Epigen

SKU: CD01855

Size:5 ug

Background:Epigen is a member of the epidermal growth factor family and is expressed by several tissues, including the testis, liver, heart and in certain tumor cells. It is strongly mitogenic for fibroblasts and epithelial cells, despite its relatively weak affinity for its main receptor, ErbB1. The mitogenic potential of Epigen is enhanced by its unusually long persistence on the membrane before ubiquitylation and receptor-mediated depletion. The gene of human Epigen encodes a 154 amino acid (a.a.) residue type I transmembrane precursor glycoprotein which contains a 22 a.a. signal peptide, an 88 a.a. extracellular domain, a 21 a.a. transmembrane domain and a 23 a.a. cytoplasmic domain. By alternative splicing of the gene, Epigen has several isoforms. The mature, shed form of human Epigen (54 – 104a.a.) shares 92 %, 94 % and 94 % a.a. sequence identity with mouse, rat and equine Epigen, respectively.

Research_area:Immunology

Purity:> 98 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Epigen

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse Balb/c 3T3 cells is less than 3 ng/ml, corresponding to a specific activity of > 3.3 × 13 IU/mg.

Synonyms:Epigen,EPGN,ALGV372, EPG, PRO994, Human

Endotoxin:Less than 1 EU/μg of rHuEpigen as determined by LAL method.

Storage:-20ºC

Sequence:AVTVTPPITA QQADNIEGPI ALKFSHLCLE DHNSYCINGA CAFHHELEKA ICRCFTGYTG ERCEHLTLTS YA

uniprot accession:Q6UW88; H3BM13;APK19

Molecular weight:Approximately 7.9 kDa monomeric protein, containing 72 amino acid residues, which comprises the EGF homologous portion of the Epigen precursor.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.