Recombinant Human DUSP3/VHR Protein (His Tag)

SKU: PKSH033476

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: DUSP3;VHR

Target Synonym: DUSP3;Dual specificity protein phosphatase 3;Dual specificity protein phosphatase VHR;VHR;Vaccinia H1-related phosphatase

Research Areas: Signal Transduction;Cancer;metabolism;

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: N-His

UNIProt ID: P51452

Accession: P51452

Background: Human DUSP3 belongs to the dual specificity protein phosphatase subfamily. DUSPs are a heterogeneous group of protein phosphatases that can dephosphorylate both phosphotyrosine and phosphoserine/phosphothreonine residues within the one substrate. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. DUSPs are major modulators of critical signalling pathways that are dysregulated in various diseases. They negatively regulate members of the mitogen-activated protein kinase superfamily; which are associated with cellular proliferation and differentiation. DUSP3 is expressed in human tissues including breast and ovarian.DUSP3 shows activity both for tyrosine-protein phosphate and serine-protein phosphate; but displays a strong preference toward phosphotyrosines. Human DUSP3 specifically dephosphorylates and inactivates ERK1 and ERK2.

Concentration:

Activity: Not validated for activity

Sequence: Ser2-Pro185

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of PBS, pH7.4.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 22.6 kDa

ObservedMW: 18-22 kDa