Recombinant Human DDIT3 Protein(GST Tag)

SKU: PDEH100664

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:DDIT3

Target Synonym:DDIT;GADD;DNA damage inducible transcript;DDIT3;CEBPZ;CHOP;CHOP-10;CHOP10;GADD153;C/EBPzeta;DDIT3 / CHOP;DNA damage inducible transcript 3;CEBPZ;CHOP;CHOP10;CHOP-10;DNA damage inducible transcript 3;GADD153;C/EBP homologous protein;C/EBP Homology Protein;C/EBP zeta;C/EBP-homologous protein;C/EBP-homologous protein 10;CCAAT/enhancer binding protein homologous protein;CHOP 10;DDIT 3;DDIT-3;DNA damage-inducible transcript 3 protein;GADD 153;Growth Arrest and DNA Damage Inducible Protein 153;Growth arrest and DNA damage inducible protein GADD153;Growth arrest and DNA damage-inducible protein GADD153;MGC4154

Research Areas:Epigenetics and Nuclear Signaling;Stem Cells;Developmental Biology;Metabolism

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:N-GST

UNIProt ID:P35638

Accession:P35638

Background:CHOP/GADD153, also known as DNA-damage-inducible transcript 3 (DDIT3), is a basic domain-leucine zipper(bZIP) transcription factor of C/EBP family. This protein has been shown to be up-regulated by several stresses, such as amino acid or glucose starvation, endoplasmic reticulum (ER) stress, osmotic stress and hypoxia. GADD153 protein may play a role in ER stress-mediated apoptosis and in disease including diabetes, brain ischemia and neurodegenerative disease. Recombinant GADD153 fused with His-tag, was expressed in E.coli and purified by conventional chromatography techniques.

Concentration:

Activity:Not validated for activity

Sequence:Met1-Ala169

Purity:> 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method

Calculated MW:34.6 kDa

ObservedMW:43 kDa