Recombinant Human CSF1R/CD115 Protein (His & GST Tag)

SKU: PKSH030422

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: C-FMS;CD115;CSF-1 receptor;CSF-1-R;CSF-1R;CSF1R;FIM2;FMS;HDLS;M-CSF-R;MCSF Receptor ;Macrophage colony-stimulating factor 1 receptor;Proto-oncogene c-Fms

Research Areas: Signal Transduction;Cardiovascular;immunology;

Conjugation:

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Application:

Fusion tag: N-His-GST

UNIProt ID: P07333

Accession: NP_005202.2

Background: M-CSFR encoded by the proto-oncogene c-fms is the receptor for colony stimulating factor 1 (CSF1R), a cytokine involved in the proliferation, differentiation, and activation of macrophages. This cell surface glycoprotein is consisted by an extracellular ligand-binding domain, a single membrane-spanning segment, and an intracellular tyrosine kinase domain. Binding of CSF1 activates the receptor kinase, leading to "autophosphorylation" of receptor subunits and the concomitant phosphorylation of a series of cellular proteins on tyrosine residues. CSF1R is a tyrosine kinase receptor that is absolutely required for macrophage differentiation and thus occupies a central role in hematopoiesis. CSF1 and its receptor (CSF1R, product of c-fms proto-oncogene) were initially implicated as essential for normal monocyte development as well as for trophoblastic implantation. This apparent role for CSF1/CSF1R in normal mammary gland development is very intriguing because this receptor/ligand pair has also been found to be important in the biology of breast cancer in which abnormal expression of CSF1 and its receptor correlates with tumor cell invasiveness and adverse clinical prognosis. Tumor cell expression of CSF1R is under the control of several steroid hormones (glucocorticoids and progestins) and the binding of several bHLH transcription factors, while tumor cell expression of CSF-1 appears to be regulated by other hormones, some of which are involved in normal lactogenic differentiation.

Concentration:

Activity: Not validated for activity

Sequence: Lys 543-Cys 972

Purity: > 82 % as determined by reducing SDS-PAGE.

Formulation: Supplied as sterile solution of 50mM Tris, 150mM NaCl, pH 7.4, 20% glycerol, 0.3mM DTT

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 76 kDa

ObservedMW: 75 kDa