Recombinant Human COL9A1 Protein (His Tag)

SKU: PKSH033337

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: COL9A1

Target Synonym: Collagen alpha-1(IX) chain;DJ149L1.1.2;DJ149L1.1.2 ;EDM6;MED;STL4

Research Areas: Signal Transduction

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: P20849

Accession: P20849-3

Background: COL9A1; which is short for Collagen alpha-1(IX) chain; is a 921 aa. protein. It is a secreted protein; and exists in extracellular space and extracellular matrix. This protein is a heterotrimer of an alpha 1(IX); an alpha 2(IX) and an alpha 3(IX) chain.Each subunit is composed of three triple-helical domains interspersed with non-collagenous domains. The globular domain at the N-terminus of type IX collagen molecules represents the NC4 domain which may participate in electrostatic interactions with polyanionic glycosaminoglycans in cartilage. It ia a structural component of hyaline cartilage and vitreous of the eye.

Concentration:

Activity: Not validated for activity

Sequence: Ala24-Pro328

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 33.8 kDa

ObservedMW: 38-50 kDa