Recombinant Human CD47/IAP Protein (His Tag)

SKU: PKSH033600

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Immune Checkpoint Proteins

Abbreviation: CD47;IAP

Target Synonym: Antigenic Surface Determinant Protein OA3;CD47;IAP;Integrin-Associated Protein;Leukocyte Surface Antigen CD47;MER6;Protein MER6

Research Areas: Cancer;immunology

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: BLI

Fusion tag: C-His

UNIProt ID: Q08722

Accession: Q08722

Background: CD47(Integrin-Associated Protein;IAP) is a 40 - 60 kDa variably glycosylated atypical member of the immunoglobulin superfamily. The ubiquitously expressed CD47 binds to SIRP family members on macrophages; neutrophils; and T cells. CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The protein is also a receptor for the C-terminal cell-binding domain of thrombospondin; and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution; and is reduced in expression on Rh erythrocytes.

Concentration:

Activity: Loaded Anti-Human CD47 mAb-Fc on Protein A Biosensor, can bind Human CD47-His with an affinity constant of 0.22 nM as determined in BLI assay.

Sequence: Gln19-Pro139

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 10mM Tris-Citrate, 150mM NaCl, pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 14.8 kDa

ObservedMW: 30-45 kDa