Recombinant Human CD32b/FCGR2B Protein (HEK293 Cells, His Tag)

SKU: PKSH031726

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: CD32;CDw32;FCG2;FCGR2B;Fc-Gamma RII-b;Fc-Gamma-RIIb;FcRII-b;IGFR2;IgG Fc Receptor II-b;Low Affinity Immunoglobulin Gamma Fc Region Receptor II-b

Research Areas: immunology;Stem cells

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion tag: C-His

UNIProt ID: P31994

Accession: NP_001002274.1

Background: Processing of the N-terminal initiator methionine or formylated methionine is an essential cellular process conserved from prokaryotes to eukaryotes. The proteolytic removal of N-terminal methionine from nascent peptides is catalyzed by a family of enzymes known as methionine aminopeptidases (MetAPs) and is essential for cell growth. METAP1 and METAP2 have different substrate specificity due to the differences in both size and shape of the active sites. As a member of the M24 family of metalloproteases, METAP1 plays an important role in G(2)/M phase regulation of the cell cycle and may serve as a promising target for the discovery and development of new anticancer agents.

Concentration:

Activity: Immobilized recombinant human CD32b at 10 μg/ml (100 μl/well) can bind human IgG2 with a linear range of 0.16-6. 4 μg/ml.

Sequence: Ala 46-Pro 217

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 20.8 kDa

ObservedMW: 25-30 kDa