Recombinant Human CD27/TNFRSF7 Protein (Fc Tag)

SKU: PKSH031924

Size: 200μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Immune Checkpoint Proteins

Abbreviation:

Target Synonym: S152;S152. LPFS2;S152.LPFS2;T14;TNFRSF7;Tp55

Research Areas: Cancer;immunology;Stem cells

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion tag: C-rFc

UNIProt ID: P26842

Accession: P26842

Background: CD27; also known as TNFRSF7; is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage; and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer; CD27 has been shown to play roles in lymphoid proliferation; differentiation; and apoptosis.It has important role in generation of T cell immunity; and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule; the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity; and meanwhile; plays a key role in regulating B-cell differentiation; activation and immunoglobulin synthesis.

Concentration:

Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD27-his at 10 μg/mL (100 μl/well) can bind biotinylated human CD70-Fc, The EC50 of biotinylated human CD70-Fc is 95-150 ng/mL.

Sequence: Met 1-Ile192

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 44.4 kDa

ObservedMW: 63 kDa