Recombinant Human Carboxypeptidase E/CPE Protein (His Tag)

SKU: PKSH032173

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: Carboxypeptidase E;CPE

Target Synonym: Carboxypeptidase E(CPE for short);Carboxypeptidase H;Enkephalin convertase;Prohormone-processing carboxypeptidase

Research Areas: Signal Transduction;Neuroscience;Cancer;metabolism;

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: P16870

Accession: P16870

Background: The active form of CPE cleaves C-terminal amino acid residues of the peptide, and is thus involved in the biosynthesis of peptide hormones and neurotransmitters including insulin, enkephalin, etc. It is thought that membrane-associated CPE acts as a sorting receptor for targeting regulated secretory proteins which are mostly prohormones and neuropeptides in the trans-Golgi network of the pituitary and in secretory granules into the secretory pathway. Defects in this protein are implicated in type II diabetes due to impaired glucose clearance and insulin resistance.

Concentration:

Activity: Not validated for activity

Sequence: Arg42-Ser453

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Histidine-HCl, 8% Sucrose, 2% Glycine, 20% Glycerol, 0.05% Tween 80, pH 6.5.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 47.2 kDa

ObservedMW: 50-60 kDa