Recombinant Human BRCA2 Protein (His Tag)

SKU: PDEH100705

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:BRCA2

Target Synonym:BRCA 2;BRCA1/BRCA2 containing complex subunit 2;BRCA2;BRCC 2;BRCC2;Brca2;Breast Cancer 2 t;Breast and ovarian cancer susceptibility gene early onset;Breast cancer 2 early onset;DNA repair associated;breast and ovarian cancer susceptibility protein 2

Research Areas:Epigenetics and Nuclear Signaling,Cancer

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:Immunogen

Fusion tag:N-His

UNIProt ID:P51587

Accession:P51587-1

Background:Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele.

Concentration:

Activity:Not validated for activity

Sequence:Thr2350-Val2545

Purity: > 85% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:22.1 kDa

ObservedMW:25.4 kDa