Recombinant Human BMP-10 protein(His Tag)

SKU: PKSH034137

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: BMP-10

Target Synonym: BMP-10

Research Areas: Signal Transduction,Cardiovascular

Conjugation:

Target Species: Human

Expression Host: E.coli

Application: Cell culture

Fusion tag: C-His

UNIProt ID: O95393

Accession: O95393

Background: Required for maintaining the proliferative activity of embryonic cardiomyocytes by preventing premature activation of the negative cell cycle regulator CDKN1C/p57KIP and maintaining the required expression levels of cardiogenic factors such as MEF2C and NKX2-5. Acts as a ligand for ACVRL1/ALK1, BMPR1A/ALK3 and BMPR1B/ALK6, leading to activation of SMAD1, SMAD5 and SMAD8 transcription factors. Inhibits endothelial cell migration and growth. May reduce cell migration and cell matrix adhesion in breast cancer cell lines.

Concentration:

Activity: Measure by its ability to induce alkaline phosphatase production by ATDC5 cells.The ED₅₀ for this effect is 1.7-2.1 ng/mL.

Sequence: Asn 317-Arg 100

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 20 mM sodium citrate, 0.2 M NaCl, pH 3.5.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 13.1 kDa

ObservedMW: 16 kDa